Abstract
The combinatorial cloning of antibody variable domains and the display of antibody fragments on the surface of filamentous phage by fusion to phage coat proteins provides an alternative strategy for the rapid generation of monoclonal antigen-binding proteins. Various antibody-displaying phage libraries have been described, which are based on the B cell repertoire of rearranged immunoglobulin genes from spleen and bone marrow of previously immunized mice (see Chapter 2, Chapter 3). However, the combinatorial library technique may also be applied for the generation of monoclonal antibody fragments from species, which are not easily amenable to conventional methodology based on eukaryotic cell fusion techniques, e.g. humans, rabbits, and chicken. The numerous reports about the isolation of functional antibody fragments from combinatorial libraries prepared from peripheral blood lymphocytes from immunized or non-immunized human donors exemplify the general applicability of this approach (see Chapter 6, Chapter 7).
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
