Abstract
The effect of in vivo administration of anti-L3T4 monoclonal antibody on the generation of an L3T4 −/Ly-2 − (CD4 −/CD8 −) population of Thy-1.2 + cells was examined in Peyer's patches of mice by two-color flow cytometry. Female BALB/c mice aged 8 wk were given 4–6 weekly injections of either anti-L3T4 (1 mg/wk) or phosphate-buffered saline (control group), and dispersed Peyer's patch cells analyzed for the presence and absence of L3T4 and Ly-2 on Thy-1.2 + cells. In anti-L3T4 treated mice, L3T4 −/Ly-2 − T cells accounted for 25–30% of the Thy-1.2 + cells, whereas in control mice these cells represented only 3–4% of the T cells. The remaining 70–75% of the Thy-1.2 + cells after anti-L3T4 treatment were Ly-2 + and not L3T4 +. The L3T4 −/Ly-2 − population of Thy-1.2 + cells is a novel subset which has not been previously found in Peyer's patches and is amplified when helper T cells are depleted.
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