Abstract
The present study generated a polyclonal antibody (AP86/3) that recognises a peptide sequence of the h5-HT 3B receptor subunit. Western blot analysis of homogenates prepared from cell lines expressing either homomeric (h5-HT 3A) or heteromeric (h5-HT 3A/3B) receptors, as well as immunocytochemical studies with the same cell lines, indicated that AP86/3 recognised, selectively, the 5-HT 3B subunit. Immunohistochemical labelling was also apparent in cells in the rat hippocampus that displayed the distribution and morphology of interneurones.
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