Clinical trials that tested the antitumor activity of γδ Tcells have been mostly unsuccessful. To address this, we expanded human Vγ9Vδ2 Tcells in TGFβ1, a cytokine which enhances their viability, trafficking properties, and intrinsic antitumor activity. This protocol summarizes the production and in vitro functional characterization of TGFβ1 educated human Vγ9Vδ2 cells and highlights their compatibility with chimeric antigen receptor (CAR) engineering. We also describe in vivo testing of the antitumor activity of these CAR Tcells in mice. For complete details on the use and execution of this protocol, please refer to Beatson etal. (2021).
Chimeric Antigen Receptor Human Vγ9Vδ2 Tcells Intrinsic Antitumor Activity Tcells In Mice Human Vγ9Vδ2 Trafficking Properties Antitumor Activity Of Tcells Human Tcells Generation Of Tcells Antitumor Activity
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Round-ups are the summaries of handpicked papers around trending topics published every week. These would enable you to scan through a collection of papers and decide if the paper is relevant to you before actually investing time into reading it.
Climate change Research Articles published between Nov 21, 2022 to Nov 27, 2022
Nov 28, 2022
Articles Included: 2
No potential conflict of interest was reported by the authors. The conception and design of the study, acquisition of data, analysis and interpretatio...Read More
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