STAR protocols | VOL. 3
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Generation and application of TGFβ-educated human Vγ9Vδ2 Tcells.

Publication Date Jun 17, 2022

Abstract

Clinical trials that tested the antitumor activity of γδ Tcells have been mostly unsuccessful. To address this, we expanded human Vγ9Vδ2 Tcells in TGFβ1, a cytokine which enhances their viability, trafficking properties, and intrinsic antitumor activity. This protocol summarizes the production and in vitro functional characterization of TGFβ1 educated human Vγ9Vδ2 cells and highlights their compatibility with chimeric antigen receptor (CAR) engineering. We also describe in vivo testing of the antitumor activity of these CAR Tcells in mice. For complete details on the use and execution of this protocol, please refer to Beatson etal. (2021).

Concepts

Chimeric Antigen Receptor Human Vγ9Vδ2 Tcells Intrinsic Antitumor Activity Tcells In Mice Human Vγ9Vδ2 Trafficking Properties Antitumor Activity Of Tcells Human Tcells Generation Of Tcells Antitumor Activity

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No potential conflict of interest was reported by the authors. The conception and design of the study, acquisition of data, analysis and interpretatio...

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