Abstract
BackgroundAdventitious root (AR) formation is a critical step in vegetative propagation of most ornamental plants, such as carnation. AR formation from stem cuttings is usually divided into several stages according to physiological and metabolic markers. Auxin is often applied exogenously to promote the development of ARs on stem cuttings of difficult-to-root genotypes.ResultsBy whole transcriptome sequencing, we identified the genes involved in AR formation in carnation cuttings and in response to exogenous auxin. Their expression profiles have been analysed through RNA-Seq during a time-course experiment in the stem cutting base of two cultivars with contrasting efficiencies of AR formation. We explored the kinetics of root primordia formation in these two cultivars and in response to exogenously-applied auxin through detailed histological and physiological analyses.ConclusionsOur results provide, for the first time, a number of molecular, histological and physiological markers that characterize the different stages of AR formation in this species and that could be used to monitor adventitious rooting on a wide collection of carnation germplasm with the aim to identify the best-rooting cultivars for breeding purposes.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-2003-5) contains supplementary material, which is available to authorized users.
Highlights
Adventitious root (AR) formation is a critical step in vegetative propagation of most ornamental plants, such as carnation
AR formation from stem cuttings is usually divided into several stages according to physiological and metabolic markers: i) dedifferentiation, during which cells become competent to respond to the rhizogenic signal, ii) cell division, and iii) root
Several complementary Deoxyribonucleic acid (DNA) (cDNA) libraries prepared from stem cutting bases of mock-treated and auxin-treated samples at particular time points during adventitious rooting (0, 6, 24 and 54 h after planting (hAP)) were sequenced
Summary
Adventitious root (AR) formation is a critical step in vegetative propagation of most ornamental plants, such as carnation. The proteomic analysis of mutants affected in AR formation led to the identification of 11 proteins, including three auxin-inducible GRETCHEN HAGEN (GH3)-like proteins, whose expression was altered [18] These GH3-like proteins are required for fine-tuning AR initiation in the hypocotyl, through modulating jasmonic acid homeostasis [19]. As an alternative strategy to identify genes involved in AR formation, a number of studies have been conducted to characterize the gene expression profiles in the stem cutting base of different species during rooting [13, 20,21,22,23,24,25] Based on these studies, some of the molecular events occurring during AR formation have been outlined. Our results will allow the identification of the genes involved in AR formation in this species, which will contribute to our basic understanding of the molecular events leading to this complex developmental response
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
