Abstract
e12503 Background: The differences in the gene expression profiles of tumor samples from primary and brain metastases have a potential predictive value in determining the patient population likely to develop brain metastases. Methods: Using isolated breast and lung samples including paired primary and metastatic tumors obtained from cancer patients, the gene expression profiles were compared. The local Institutional Research Board approval was obtained to use the biopsy specimens and clinical data. Following laser-capture micro-dissection, RNA isolation and amplification, gene expression of the microdissected biopsy specimens was evaluated using HG-U133 microarrays. Of the ten identified cases with paired primary and brain metastases, seven paired cases of breast (n = 2) and lung (n = 5) were fully analyzed. Selected genes with significantly different expressions were validated by RT-PCR using cDNA generated from RNA isolated from tumor cells microdissected from an independent set of breast (n = 12) and l...
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
