Abstract
ABSTRACTThe expression of gelsolin (GSN) is abnormal in many cancers, including extranodal nasal-type natural killer/T-cell lymphoma (NKTCL). However, the biological function of GSN and its mechanism in NKTCL remain unclear. We found that GSN overexpression significantly suppressed cell proliferation, colony formation and invasion, and promoted apoptosis of natural killer (NK) cell line YTS. Moreover, the upregulation of GSN significantly decreased the levels of PI3K and p-Akt. Interestingly, blocking the PI3K/Akt signaling pathway significantly inhibited cell proliferation and invasion and promoted apoptosis of YTS cells. In conclusion, our findings indicate that GSN can suppress cell proliferation and invasion and promote apoptosis of YTS cells, and the PI3K/Akt signaling pathway is likely to be involved in this process.
Highlights
Extranodal nasal-type natural killer/T-cell lymphoma (NKTCL) is one of the Epstein-Barr virus (EBV)-related hematological malignancies, which mainly develops in the nasal cavity but can occur in extranasal sites, either as a primary extranasal or disseminated disease (Harabuchi et al, 1996; Chen et al, 2015)
Overexpression of GSN in transfected YTS cells After the lentivirus-containing Lenti-Con and Lenti-GSN vectors were transfected into natural killer (YTS) cells, green fluorescence was obvious in the infected YTS cells, as observed under a fluorescence microscope, and the result indicated a successful transfection
Real-time quantitative reverse transcriptase polymerase chain reaction analysis and western blot analysis demonstrated that the mRNA and protein levels of GSN were both significantly increased in the YTS cells transfected with the pCDH-CMV-MCS-EF1-copGFP-GSN vector (YTS-GSN cells), when compared with the YTS cells transfected with the pCDHCMV-MCS-EF1-copGFP vector (YTS-Con cells) (Fig. 1C,D)
Summary
Extranodal nasal-type natural killer/T-cell lymphoma (NKTCL) is one of the Epstein-Barr virus (EBV)-related hematological malignancies, which mainly develops in the nasal cavity but can occur in extranasal sites, either as a primary extranasal or disseminated disease (Harabuchi et al, 1996; Chen et al, 2015). NKTCL is more common in Asia than in Western countries (Au et al, 2009). Most of the cases of NKTCL are diagnosed in the early stage of the disease, the long-term survival rate of patients is ∼46%-60% (Suzuki et al, 2010). The one-year survival rate of patients with advanced-stage disease is only 50%, despite improvements in treatment (Jaccard et al, 2011; Yamaguchi et al, 2011). The tumor cells of NKTCL derived from NK cells and, rarely, T cells are linked to EBV infection (Huang et al, 2013). The biological characteristics of NKTCL are not yet completely clear
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