Gelatinase activities and haptoglobin concentrations in healthy and in degenerative articular cartilage of pigs

Abstract

The objectives of this study were to investigate the activity of the matrix metalloproteinase (MMP)-2 and MMP-9 in healthy and in degenerative cartilage and to characterize the relation with the acute phase protein haptoglobin (HP) in articular cartilages of pigs. Joint surfaces of the proximal and distal humerus and femur of fattening pigs were histopathologically classified. In addition, cartilage homogenates and synovia were obtained. The tissue homogenates were analysed for gelatinase activity by zymography and by activity assay. The concentrations of HP in cartilage homogenates, in synovia and in serum were analysed by ELISA. High enzymatic activity of the MMP-2 latent form was observed in zymography in all samples. Zymographic activities of MMP-2 active form and MMP-9 (active and latent form) were detected at low levels in some samples. Comparison of the zymographic activities of gelatinases in unaltered vs. altered cartilages yielded no differences. In contrast to zymography, cartilage homogenates were negative for MMP-2 and MMP-9 in the activity assays. The concentrations of HP in cartilage homogenates and in synovia from samples without alteration and from samples with massive alterations were not different. When classified according to their HP concentration, cartilage homogenates with increased HP concentrations had higher (p < 0.05) zymographic activities of the MMP-2 active form. For the two MMPs investigated, there was no detectable relationship with degenerative processes in the cartilage.