Abstract
Universal calibration studies show that several cationica polyelectrolytes can be characterized by aqueous exclusion chromatography, without adsorption effects using PW (Toyo Soda) hydrophilic gel columns. No. other commercially available, high-performance packings are suitable for polycations, primarily because of charge-induced adsorption. In the case of the present columns, such interactions are minimized by 0.2 M NaCl in the mobile phase, which also provides an ionic strength sufficient to suppress Donnan equilibrium salt peaks.
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