Abstract
Strobilanthes kunthiana (Neelakurinji) is an endemic and underexploited plant belongs to the Family Acanthaceae. The aim of our study was to evaluate phytochemical analysis, GC-MS analysis and antioxidant activity of in vitro callus extract of Strobilanthes kunthiana. In this present study the phytochemical analysis of various extract of S. kunthiana in vitro callus were studied. Phytochemical analysis confirmed the presence of alkaloids, flavonoids, glycosides, saponins, tannins, steroids, terpenoids and phenols in the methanolic extract comparing to the other extracts. In this study GC-MS analysis revealed the presence of 10 bioactive phytochemical compounds were identified in the methanolic extract. The prevailing compounds were 9,12-octadecadienoic acid (Z,Z) (50.32%), hexadecanoic acid, methyl ester (20.69%), 9-octadecenoic acid (Z)-,methyl ester (10.45%), heptadecanoic acid, 16-methyl-, methyl ester (5.78%), 2,6-bis(1,1-dimethylethyl)-4-methyl phenol (4.70%). 3-methyl-2-ketobutyric acid tbdms (2.79%), 2,2,3,4-tetramethyl-5-hexen-3-ol (2.41%), N-(tert-butoxycarbonyl)-2-(4-methoxyphenyl) allylamine (1.06%), cyclotrisiloxane, hexamethyl (0.94%), benzenesulfonamide (0.87%). The antioxidant property was evaluated for methanol and ethanolic extract by DPPH method. The higher percentage of inhibition (79.23 +/- 0.37) was observed in 250 μg/mL of ethanol extract followed by (90.35 +/- 0.54) methanolic extract against the standard ascorbic acid (91.25 +/- 0.33). The results show that the methanolic extract possesses more antioxidant activity than ethanol. The plant S. kunthiana may be exploited as a source of natural antioxidant and as herbal alternatives for various disorders.
Highlights
India has a rich culture of medicinal herbs which includes about8,000 species of known medicinal plants and about more than 2,000 species and has a vast geographical area with high potential abilities for Ayurvedic, Unani, ISSN 2358-2731/BJBS-2018-0024/5/10/15/359Braz
The results show that the methanolic extract possesses more antioxidant activity than ethanol
Analysis, antioxidant activity of in vitro callus of Strobilanthes kunthiana have been studied by following methods as given below
Summary
India has a rich culture of medicinal herbs which includes about8,000 species of known medicinal plants and about more than 2,000 species and has a vast geographical area with high potential abilities for Ayurvedic, Unani, ISSN 2358-2731/BJBS-2018-0024/5/10/15/359Braz. Plants have been provided mankind with sources of medicinal agents, natural products, once serving as the source of all drugs (Balandrin et al, 1993). It plays an important role in searching for novel drugs based on their new modes of pharmacological actions. This action may due to the presence of carbohydrates, carbohydrate derivatives, gums, mucilage’s, pectin’s, glycosides tannins, phenolic compounds, lipids, fixed and volatile oils, resins, alkaloids, terpenoids flavonoids, steroids etc
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