Abstract
A miniature permeation device is used to add controlled amounts of gas-phase dopant to the air around two types of atmospheric pressure nanoflow electrospray source: a pulled glass capillary needle in a custom mount, and for the first time a commercial nano-liquid chromatography emitter in a commercial ion source housing. Intact proteins sprayed at 300 nL/min are charge-reduced by the addition of nitrogen doped with diethylamine, and the reaction is assisted by adjusting flow rate, positioning, and enclosure of the nanoflow emitter. Charge distribution is shifted to lower charge states with increasing amounts of dopant. The maximum signal of the most abundant charge state and the total signal abundance are increased to an extent dependent on the mass and isoelectric point of the protein.
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