Abstract
A method is proposed for the analysis of small amounts of complicated natural mixtures of fatty acids that are not chemically bonded in lipids. The analysis includes isolation of free fatty acids, formation of methyl esters, preparative gas chromatographic separation, hydrogenation of double bonds and analysis with a system of specially chosen columns. The method was used successfully in the analysis of free fatty acids in salmon flesh in which 52 components were found and 43 were identified, consisting of odd- and even-carbon-numbered acids with 12–24 carbon atoms, 0–6 double bonds and also branched chains.
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