Abstract
This research aimed to determine the effect of willow (Salix tetrasperma Roxb) tree bark extract on histopathological changes of liver of rat (Rattus novergicus) infected with Trypanosoma evansi. Liver of 25 male rat, Wistar strain, were used in this study and all rat were fed with commercial feed ad libitum. This research used completely randomized design (CRD) with 5 treatments, each treatment consisted of 5 replicates. Treatment I was negative control group without any treatment, treatment II were only infected with 103 T. evansi, treatment III, IV, and V were infected with 103 T. evansi and treated with willow tree bark extract with the dose of 30, 45, and 60 mg/kg bw, respectively. The administration of willow tree bark extract was carried out for 3 consecutive days orally using a stomach tube. All rats were sacrificed and necropsied then liver were collected for histopathological examination. Percentage of histophatological changes found in hepatocytes of rat infected with T. evansi after the administration of willow tree bark extract in group P1, P2, P3, P4, and P5 were: normal hepatocytes 96.28%; 0.00%; 8.06%; 40.61%; and 0.00%; hydropic degeneration 0.00%, 44.98%, 58.00%, 38.29%, and 35.04%; fatty degeneration 0.00%, 0.00%, 0.00%, 0.00%, and 8.76%; necrosis 3.72%, 55.02%, 33.94%, 21.10%, and 56.20, respectively. In conclusion, the administration of willow tree bark extract is able to protect rat’s liver from T. evansi infection and the dose of 45 mg/kg bw is more effective than 30 mg/kg bw but the dose of 60 mg/kg bw of willow tree bark extract worsen the liver damage.Key words: histopathology, liver, T. evansi, willow
Highlights
This research aimed to determine the effect of willow
all rat were fed with commercial feed ad libitum
each treatment consisted of 5 replicates
Summary
Infeksi T. evansi sangat merugikan peternak karena dapat menyebabkan penurunan berat badan, daya reproduksi yang rendah, dan kematian (Luckins, 1996; Astuti et al, 2007). Obat trypanosida yang sudah digunakan untuk mengobati penyakit sura di berbagai negara adalah suramin, diminazene aceturat, isometamidium, quinapyramine, dan cymelarsan. Pada masa yang akan datang pengendalian penyakit ini tergantung pada kemampuan obat antitrypanosoma yang baru. Hal ini dikarenakan ekstrak kulit batang jaloh efektif terhadap Plasmodium (Asmilia, 2010). Hasil penelitian Rafina (2014), infeksi T. evansi berpengaruh terhadap gambaran darah tikus yaitu terjadi penurunan nilai packed cell volume (PCV) dan eritrosit, sedangkan leukosit mengalami peningkatan. Pemberian ekstrak kulit batang jaloh dengan dosis tinggi dapat meningkatkan nilai PCV dan eritrosit sedangkan nilai leukosit menurun pada dosis yang rendah. Penelitian ini bertujuan mengetahui pengaruh ekstrak kulit batang jaloh terhadap pencegahan kerusakan hati akibat infeksi T. evansi pada tikus. Dari hasil penelitian ini diharapkan dapat memberikan informasi mengenai kemampuan ekstrak kulit batang jaloh dalam mencegah kerusakan hati akibat infeksi T. evansi
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