Abstract
Lectins may function as pattern-recognition receptors (PRRs) and play important roles in immune responses. In this study, a novel galectin-1 (designated OfGLec-1) gene was identified from rock bream, Oplegnathus fasciatus, cDNA library. The complete cDNA sequence of the OfGLec-1 gene is 993 base pairs in length, and encodes 135 amino acids. OfGLec-1 shares a highly conserved carbohydrate recognition domain with galectin-1 from other fishes. The mRNA expressions of OfGLec-1 in healthy and bacterial and viral challenged rock breams were examined using quantitative real time polymerase chain reaction (qRT-PCR). OfGLec-1 is highly expressed in head kidney and other tissues of the rock bream. The expression is strongly up-regulated after bacterial and viral challenge. To understand its biological activity, the recombinant OfGLec-1 gene was constructed and expressed in Escherichia coli BL21 (DE3). The recombinant OfGLec-1 specifically agglutinated including E. coli, Vibrio alginolyticus and Edwardsiella tarda at a concentration of 50 μg/mL in a calcium independent manner. Both the gene expression analysis and the recombinant protein activity assay suggest that OfGLec-1 is an important immune gene involved in the recognition and elimination of pathogens in rock breams.
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