Abstract
The article shows the results concerning the problem of the influence of the hormonal composition of the medium on callus induction in isolated from different explants of Egyptian henbane areas (on the example of Hyoscyamus muticus L.). The authors study 11 variants of Murashige and Skoog medium supplemented with different concentrations and combination of auxins and cytokinins. It was important to find nutrient medium modification of Murashige and Skoog for callus induction. The article describes the fact that callus formation from different explant types of Hyoscyamus muticus L. in vitro was observed on Murashige and Skoog medium fortified with benzylaminopurine and naphthylacetic acid. It shows that the maximum callus induction was observed from root explants on Murashige and Skoog's medium supplemented with 0.5 mg/l of benzylaminopurine and 1.0 mg/l of naphthylacetic acid. And minimal callus formation was observed in the area with benzylaminopurine. Callus induction of leaf and stem explants both on the hormone-free nutrient medium and with the benzylaminopurine only was not observed. Thus, the results show that the frequency of callus formation with culturing root segment is higher compared to leaf and stem segment explants (on the example of Egyptian henbane in culture in vitro). This work aims to inducing callus formation from various explants of Egyptian henbane, which can be used for plant regeneration or as a source for in vitro production of secondary metabolites.
Highlights
The results show that the frequency of callus formation with culturing root segment is higher compared to leaf and stem segment explants (on the example of Egyptian henbane in culture in vitro)
Рисунок 1 – Каллусообразование из корневых эксплантов на среде Мурасиге и Скуга (МС), дополненной 0,5 мг/л БАП в сочетании с 1,0 мг/л нафтилуксусной кислоты (НУК) Таким образом, проведенные экспериментальные исследования показали, что индукция образования и интенсивность роста каллусной ткани H. muticus in vitro зависела от регуляторов роста и типа экспланта
Summary
ПЕРСПЕКТИВНОСТЬ ИСПОЛЬЗОВАНИЯ РЕГУЛЯТОРОВ РОСТА РАСТЕНИЙ НА ИНДУКЦИЮ КАЛЛУСОГЕНЕЗА РАЗЛИЧНЫХ ТИПОВ ЭКСПЛАНТОВ HYOSCYAMUS MUTICUS L. Установлено, что на среде Мурасиге и Скуга содержащей бензиламинопурин в сочетании с нафтилуксусной кислотой наблюдалось каллусообразование из разных типов эксплатов Hyoscyamus muticus L. in vitro. Что максимальная индукция каллусогенеза из корневых эксплантов, наблюдалась при использовании среды Мурасиге и Скуга, дополненной 0,5 мг/л бензиламинопурином и 1,0 мг/л нафтилуксусной кислотой, тогда как минимальное каллусообразование наблюдалось на среде, содержащей только бензиламинопурин.
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