Abstract
AbstractThe factor (s) responsible for rescue of the SV40 genome from transformed cells (activator) is essentially detected in nuclear extracts from permissive cells. Removal of nucleic acids from these extracts improves their activating capacity on transformed cells. By increasing the yield of infectious viral DNA, virions become frequently detectable. For a given experiment, a correlation is obtained between the protein concentration of the extract and the yield of infectious SV40 DNA. Unlike nuclear extracts from normal or SV40‐transformed permissive cells, those from normal nonpermissive cells are ineffective in rescuing the viral genome. The biological activity of the factor(s) is preserved at −70° C for at least 3 months.
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