Abstract
The present paper presents a gliadin detection method. This method is based on a modified Ti electrode. Modification was performed by a simple and cheap anodization. Then, a layer of graphene oxide was added, and gliadin antibody was fixed on the electrode surface. Using this complex system, electrochemical impedance spectroscopy was used for gliadin detection. Solutions with known gliadin (a fraction from gluten) content were used for analysis. Impedance measured at a certain frequency and coating resistance were analyzed. Better results (good linearity and lower detection limit) were obtained by plotting impedance at a certain frequency versus gliadin concentration. Coating resistance was proved to be in linear dependency with gliadin concentration only at lower concentrations. This system based on titanium nanostructured electrode has the potential to be used for gluten contamination detection from foods.
Highlights
Celiac disease is a well-known chronic immune-mediated disorder initiated by dietary gluten, having a prevalence of approximately 1% worldwide [1,2,3]
A complex electrode modification was obtained on titanium substrate
To determine the ability of this system to be used for gluten detection from foods, antigliadin antibody was immobilized on electrode surface using a chemical linker NHS:EDC
Summary
Celiac disease is a well-known chronic immune-mediated disorder initiated by dietary gluten, having a prevalence of approximately 1% worldwide [1,2,3]. Research is being done, a life-long gluten-free diet, first treatment from more than a half-century, remains the only treatment currently recognized [3,4]. Adherence to a gluten-free diet prevents further complications. There some other related gluten disorders in which adherence to a gluten-free diet is required [5,6]. Barley, oats, rye or their crossbred varieties contain gluten [7]. Gluten has two subfractions: gliadins, single-chain proteins soluble in alcoholwater mixture and glutenin, insoluble poly-peptide chain macromolecules [2,8]
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