Abstract
Surface modification of bioceramic materials by covalent immobilization of biomolecules is a promising way to improve their bioactivity. This approach implies the use of organic anchors to introduce functional groups on the inorganic surface on which the biomolecules will be immobilized. In this process, the density and surface distribution of biomolecules, and in turn the final biological properties, are strongly influenced by those of the anchors. We propose a new approach based on Raman 2D mapping to evidence the surface distribution of organosilanes, frequently used as anchors on biomaterial surfaces on hydroxyapatite and silicated hydroxyapatite ceramics. Unmodified and silanized ceramic surfaces were characterized by means of contact angle measurements, atomic force microscopy (AFM) and Raman mapping. Contact angle measurements and AFM topographies confirmed the surface modification. Raman mapping highlighted the influence of both the ceramic’s composition and silane functionality (i.e., the number of hydrolysable groups) on the silane surface distribution. The presence of hillocks was shown, evidencing a polymerization and/or an aggregation of the molecules whatever the silane and the substrates were. The substitution of phosphate groups by silicate groups affects the covering, and the spots are more intense on SiHA than on HA.
Highlights
In the field of bone surgery, the treatment of pathologies such as the repair of large osseous defects, still requires a satisfactory solution [1]
This study proposes an approach based on Raman mapping to chemically characterize a silanized surface and highlight the influence of the silane functionality and the chemical composition of the substrate on the surface coverage
The surface of inorganic bioceramics can be functionalized by adsorption or by covalent grafting of organic biomolecules
Summary
In the field of bone surgery, the treatment of pathologies such as the repair of large osseous defects, still requires a satisfactory solution [1]. To overcome this limitation, research efforts are currently being made to improve the performances of synthetic bone graft substitutes. Several strategies are being developed to increase their biological performances including the substitution of various ionic species in the apatitic structure [6] In this field, silicon is known to influence the strength, the formation and the calcification of the bone tissue [7,8], and this has led to the development of silicon containing biomaterials [9].
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
