Abstract
Expression of the Saccharomyces cerevisiae proteasomal genes is regulated coordinately by ScRpn4 transcription factor and its binding site known as PACE. Genomic analysis demonstrates that only Saccharomycetes yeasts carry ScRpn4-orthologous genes and PACE-like elements in the promoters of proteasomal genes. This taxonomic group includes species of biotechnological importance such as Komagataella (Pichia) pastoris and Yarrowia lipolytica. A literature search has failed to identify studies focused on ScRpn4 orthologs of these yeast species. In the present work, using the genetic background of S. cerevisiae, we showed that ScRpn4 orthologs from K. pastoris and Y. lipolytica restore the mRNA levels of proteasomal genes and confer stress tolerance in a S. cerevisiae strain with knockout of the SCRPN4 gene. In other words, these proteins are functionally similar to ScRpn4 of S. cerevisiae.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
