Full‐length plasma skeletal muscle myosin isoform deficiency is associated with coagulopathy in acutely injured patients

Abstract

BackgroundSkeletal muscle myosin (SkM) molecules are procoagulant both in vitro and in vivo. The association of plasma SkM isoforms with blood coagulability and hemostatic capacity has not been defined. ObjectivesWe hypothesized that coagulopathy in acutely injured patients is associated with procoagulant plasma SkM heavy chain levels. MethodsTo test this hypothesis, citrated whole blood and plasma from 104 trauma patients were collected and studied to obtain data for rapid thrombelastography, international normalized ratios, and plasma SkM levels. Coagulability parameters were dichotomized by the threshold for the hypercoagulable trauma‐induced coagulopathy. ResultsLower plasma full‐length SkM heavy chain (full‐SkM) levels were associated with higher international normalized ratio values (>1.3) (p = .03). The full‐SkM levels were also associated with a lower rate of clot propagation (thrombelastography angle <65°) (p = .004), and plasma full‐SkM levels were positively correlated with the thrombelastography angle (r2 = .32, p = .0007). The trauma patient group with the lower plasma full‐SkM levels showed an association with lower clot strength (maximum amplitude <55 mm) (p = .002), and plasma full‐SkM levels positively correlated with maximum amplitude (r2 = .27, p = .005). Hyperfibrinolysis was associated with significantly decreased full‐SkM levels (p = .03). Trauma patients who required red blood cells and fresh frozen plasma transfusions had lower plasma full‐SkM levels compared with those without transfusions (p = .04 and .02, respectively). ConclusionsIn acutely injured trauma patients, lower levels of plasma full‐SkM levels are linked to hypocoagulability in trauma‐induced coagulopathy, implying that SkM plays a role in the hemostatic capacity in trauma patients and may contribute to trauma‐induced coagulopathy.