Abstract
BackgroundCryopreservation and transplantation of ovarian tissue is one option for re-establishing ovarian function, but optimal conditions for graft sustainment and follicular survival are still considered experimental. The present study aims to analyze the effect of FSH treatment on the resting follicle pool in fresh and cryopreserved primate ovarian tissues following xenografting.MethodsOvarian tissues from adult marmosets were grafted freshly or following cryopreservation to ovarectomized nude mice treated with FSH 25 IU twice daily post transplantation or left untreated as controls. Grafts were retrieved 2 or 4 weeks after transplantation to evaluate the number and morphological appearance of follicles.ResultsEarly start of FSH treatment within 1 week following transplantation partly prevents primordial follicle loss in fresh and frozen-thawed tissues, whereas after a 3 weeks time interval this effect is present only in fresh tissues. A similar positive effect of early, but not later FSH treatment on primary follicles is seen in fresh tissues compared to only marginal effects in frozen-thawed tissues. The percentage of morphologically normal follicles is generally increased in FSH treated tissues, whereas the percentage of primary follicles over all primordial and primary follicles is increased by FSH only in freshly-grafted tissues.ConclusionsFSH treatment alleviates depletion of the resting follicle pool and promotes normal follicular morphology both in freshly and frozen-thawed grafted tissues. In previously cryopreserved tissues, applying to most of the tissues intended for clinical use in fertility preservation attempts, its positive effect on primordial follicle numbers and potential graft sustainment is dependent on an early start of treatment within one week of transplantation.
Highlights
Cryopreservation and transplantation of ovarian tissue is one option for re-establishing ovarian function, but optimal conditions for graft sustainment and follicular survival are still considered experimental
We have previously evaluated the effect of cryopreservation on marmoset ovarian tissue using a xenograft model [8], demonstrating resumption of follicular growth and sustained graft viability [9]
The present study aims to analyze the effect of FSH treatment on the resting follicle pool in fresh and cryopreserved primate ovarian tissues following xenografting
Summary
Cryopreservation and transplantation of ovarian tissue is one option for re-establishing ovarian function, but optimal conditions for graft sustainment and follicular survival are still considered experimental. Cryopreservation of ovarian tissue is one option to preserve fertility from the potentially harmful iatrogenic effects of chemotherapy or irradiation: it bears the advantage of a large follicular number to be stored for future re-establishment of ovarian function both in reproductive and endocrinological terms [2]. Potential long-term survival of grafted tissue has been documented [3,4] Despite these promising results from a few centers with expertise, conditions of tissue preparation, storage and usage still require further research. The marmoset as a non-human primate model can further promote success in cryopreservation of human ovary tissues for fertility preservation, as the accessibility of human ovarian tissue for experimental studies is highly limited and data are physiologically closer to humans than those retrieved in the more common murine models
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