Abstract

The role of nitrogenous components in malt and wort during the production of beer has long been recognized. The concentration and range of wort amino acids impact on ethanolic fermentation by yeast and on the production of a range of flavour and aroma compounds in the final beer. This review summarizes research on Free Amino Nitrogen (FAN) within brewing, including various methods of analysis.

Highlights

  • IntroductionOur understanding of the connection with yeast is relatively recent, starting with Leeuwenhoek’s microscope observations in the 17th century followed by the work of Lavoisier, Gay-Lussac, Schwann and others during the 18th and 19th centuries

  • The earliest written account of brewing dates from Mesopotamian times [1]

  • The The uptake and utilization of nitrogen arginine is asdependent a source ofon nitrogen is induced by the presence metabolism of assimilated amino the phase of the fermentation and on the total quantity provided in the wort

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Summary

Introduction

Our understanding of the connection with yeast is relatively recent, starting with Leeuwenhoek’s microscope observations in the 17th century followed by the work of Lavoisier, Gay-Lussac, Schwann and others during the 18th and 19th centuries It was not until the late 19th century that Pasteur demonstrated that fermented beverages result from the action of living yeast’s transformation of glucose (and other sugars) into ethanol [2,3,4]. Is a measure of the nitrogen compounds that may be assimilated or metabolised by yeast during fermentation. These include individual wort alpha amino acids (with the exception of proline, which is not an α-amino acid), ammonia and small peptides (di- and tri-peptides). For the purpose of this review, FAN will be used as the relevant term

Sources of Nitrogen
Wort Nitrogen
Amino Acid Uptake and Metabolism during Wort Fermentation
Peptide Uptake
Impact of Wort Nitrogen
Erlich Pathway
Measuring Wort Nitrogen
Findings
Summary

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