Formulation and Characterization of Amomum Compactum Sol. Ex Maton Nano Emulsion and Its Antibacterial and Wound Healing Activity

Abstract

Natural treatment is plant extract nanoemulsion. Forming nanoemulsions with a specific method may help skin wound healing. Fruit cardamom (Amomum compactum Sol. Ex Maton) was used as an antimicrobial, but its wound-healing activity was unstudied. This study compares nanoemulsion from cardamom seed ethanol extract to cardamom extract in suppressing bacterial growth, wound healing, and determining component makeup. Research methods with Lab experiments involve sampling, processing, extracting, and UV-VIS spectrophotometer testing secondary metabolite chemicals, creating nanoemulsions, and characterizing them with HPLC, FTIR, UV-VIS, and PSA. TPC and disc diffusion for antibacterial and wound healing characterization and SPSS analysis. The research results found high quantities of gallic acid, catechin, and epigallocatechin were found in 20% (v/v) cardamom extract nanoemulsion, with HPLC analysis showing levels of 4590.10 µg/mL, 2128.95µg/mL, and 755.42µg/mL, respectively. FTIR and UV-Vis tests indicate a single peak around the phenol compound's absorption area and describe its group by absorbing functional groups in the IR spectrum. Nanoemulsion suppressed E. coli and S. aureus better than cardamom extract. At 1% (v/v), nanoemulsion heals wounds faster than cardamom extract and gentamicin sulfate. The particle size of 53.13 ± 1.25 nm and particle size distribution of 0.56 ± 0.02 nm contribute to the stability and high activity of the nanoemulsion.