Formulation and Antioxidant Activity Analysis of Jotang Herb (Acmella paniculata) Extract Mask Cream

Flavonoid content in basil leaves can be used as an antioxidant. Serum is a preparation that is formulated from high concentrations of active substances so that the effect is absorbed more quickly by the skin. The purpose of this study was to formulate basil leaf extract serum as an antioxidant using an experimental method. Evaluation test and antioxidant test using basil leaf extract concentrations of 0.06%, 0.30%, 0.6%. Evaluation of serum preparations was carried out with parameters namely organoleptic test, homogeneity test, pH test, spreadability test, Viscosity test, stability test, and antioxidant test with the DPPH method. The results showed that after stability testing for 6 cycles the serum formula did not change color, homogeneous conditions, the average formula pH was F1 (4.659 ± 0.007), F2 (4.826 ± 0.002), F3 (4.981 ± 0.001), the viscosity of the preparation F1 ( 2356 ± 40.412 cps), F2 (2285 ± 5 cps), F3 (2176 ± 1.572 cps), the average spread of the preparation was 5.2-6.3 cm. it was concluded that the serum formula gave good results and fulfilled the parameter requirements for evaluating the physical quality of the preparation. The antioxidant test results of the serum basil leaf extract formula had IC50 values in F1 (94.605 µg/mL), F2 (74.900 µg/mL), F3 (54.695 µg/mL) the three formulas were categorized into strong antioxidants.

White Pomegranate (Punica granatum L.) is a plant that is efficacious as an antioxidant which functions as a natural antioxidant to prevent free radicals from harming the body because it contains flavonoid compounds including flavones and flavonols which are known as natural antioxidants. This study aims to determine the IC50 value of extracts and formulations of white pomegranate skin sunscreen cream (Punica granatum L.) along with its SPF value. The extract was prepared by maceration using 96% ethanol. Testing the antioxidant activity of white pomegranate peel extract (Punica granatum L.) using UV-Vis spectrophotometry with the DPPH method (1,1-Diphenyl-2-Picrylhyrdazyl), the test results showed that white pomegranate peel extract (Punica granatum L.) had an IC50 value of 92,76 ppm in the strong category. Sunscreen cream was made into four formulas, namely F0, FI, FII and FIII with extract concentrations of 5%, 10% and 15% respectively. The physical evaluation was tested, including organoleptic test, homogeneity test, cream type test, spreadability test, adhesion test, pH test, viscosity test and organoleptic stability test which showed that the four formulas met the requirements of the physical evaluation test for sunscreen cream preparations. The four sunscreen cream formulas, an antioxidant activity test was carried out using the DPPH method (1,1-Diphenyl-2-Picrylhyrdazyl) at F0, FI, FII and FIII and obtained IC50 values of 146,9 ppm (weak), 98,22 ppm (strong), 81,37 ppm (strong) and 76,88 ppm (strong). Determination of the SPF (Sun Protection Factor) value was carried out using the mansyur equation method using UV-Vis spectrophotometry at a wavelength of 290-320 nm. The results obtained for the SPF values in the four formulas F0, FI, FII and FIII were 6,02 (extra), 14,39 (maximum), 22,98 (ultra) and 27,60 (ultra).

Exposure to intense and prolonged solar ultraviolet rays can cause erythema, premature aging, and skin cancer. The prevention of ultraviolet rays can be done by using sunscreen. Red Pidada leaves (Sonneratia caseolaris L.) have sunscreen properties. The purpose of this study was to preformulate red Pidada leaves into sunscreen cream preparations with the best activity as a cosmetic product prototype. This research will be started with the preparation of the red Pidada leaf ethanol extract formulated into a cream preparation with the extract concentration of Formula 1 (8%), Formula 2 (15%), and Formula 3 (20%). Evaluation of cream preparations includes organoleptic test, pH test, homogeneity test, dispersion test, adhesion test, viscosity test, and sunscreen effectiveness test. Data analysis used the One Way ANOVA test. The results of the physical quality test of cream showed that Formula 1 (8%) and Formula 2 (15%) met the requirements of the organoleptic test, homogeneity test, and adhesion test but did not meet the pH test, viscosity test, and spread ability test. Formula 3 (20%) fulfils the organoleptic test requirements and adhesion test requirements but does not meet the homogeneity test, pH test, viscosity test, and spread ability test. The sunscreen effectiveness test in formula 1 has the lowest Sun Protection Factor (SPF) value of 1.5 (without protection), formula 2 has an SPF value of 2.9 (minimum protection), and formula 3 has the highest SPF value of 7.4 (protection extra). Analysis of bioactive compounds in red Pidada ethanol extracts using LC-HRMS. There were 6 active compounds. Diisobutylphtalat, luteolin, apigethrin, vitexin, choline and bethain. Formula 3 performs better as a sunscreen than Formulas 1 and 2.

Stability is the ability of a dosage product to maintain its original properties during the storage period. Stability tests of body scrub need to be carried out to ensure the quality, safety and effectiveness with specifications and stability during storage. The purpose of this study was to determined physical stability of patchouli oil body scrub cream preparations. Patchouli oil body scrub made with variations of stearic acid FI 10 g, FII 12 g, FIII 14 g, FIV 16 g and FV 18 g were tested for physical characteristics including homogeneity and organoleptic test, which were analyzed descriptively. While the test data for pH, viscosity, and spreadability test were analyze using One-Way ANOVA analysis. Evaluation of physical stability patchouli oil body scrub for 28 days on the day before storage, 7, 14, 21, 28 of storage at 25°C/60% RH. The results organoleptic and homogeneity tests showed color remained white, had the aroma of patchouli oil, semi-solid and homogeneous. Cream type test has M/A cream type results. Spreadability result showed a range of 3,3-4,0, pH test result ranged from 6,85-7,14, viscosity tests had a range of 22.177-44.900 cP and centrifugation test result showed no separation of the two phases during 28 days of storage. According to the findings of this research, the formulation of F1 with stearate acid 10% was appropriate with better testing than another formulas, as evidenced by its spreadability, pH, mechanical study, homogenity, and viscosity there was not any change between day 0 and day 28.

Carbopol 940 is a gelling agent that is very communly used in cosmetic production because of its high compatibility and stability, and it is non-toxic when applied to the skin and it spreads on the skin more easilym gels are semi solid preparations that contain a gel-forming agent which gives stiffiness to collodial solutions or dispersions used for external use on the skin, gel preparations are widely chosen because they are very easy to apply ( easily smeared, absorbed, and cleaned) and more attractive (transparent) compared to the other topical preparations. the purpose of this study was to determine the effect of carbopol concentration 940 as a gelling agent on the physical stability of aloe vera gel preparations. this study began with optimization of the carbopol 940 gel base then the preparation formulation used 50% aloe vera extract, 0,5% carbopol 940 F1, 1 % F2, 2% F3, 10 % propylene glycol, 10% glycerine, 0,1% dm dm hydantoin and sufficient aquadest, afterward the evaluation of the preparation included organoleptic, observations, homogeneity tests, pH tests, spreadability tests, adhesion tests and viscosity tests. carbopol 940 was depeloped using co-free water. gel preparations were 0,5%, 1%, and 2%. the results showed that the thrid formulation (F3) with a concentration of 2% fulfilled the physical evaluation requirements for organoleptic, spreadability, adhesion, viscosity, pH and homogeneity tests.

Face mist is one of the cosmetics in the form of a spray, which functions as a refresher and moisturizer with the use of being directly sprayed onto the facial skin. Combining two antioxidants that work to increase the activity of preventing the effects of radiation and free radicals. Face mist is made from a combination of pomegranate peel extract (Punica granatum L.) and mangosteen peel (Garcinia mangostana L.).To obtain a formulation and evaluate the physical stability of apreparation with a face mist combination of pomegranate peel extract (Punica granatum L.) and mangosteen peel (Garcinia mangostana L.) as an antioxidant.Experimental research with One-Shoot Case Study design. Made three combination formulations of pomegranate peel and mangosteen peel extract. Then the formulation was seen from the results of the evaluation of its physical stability including organoleptic tests, homogeneity, pH, viscosity, facial moisture, spraying patterns, dry time, and antioxidant tests using the DPPH method. Analysis data One Way Anova showed that there was no significant difference in the pH, viscosity and skin moisture test. Evaluation and physical stability 21 days organoleptic test, homogeneity obtained all formulas meet the standards, pH test and dry time formula II most meet the standards, viscosity test formula III most meet the standards, formula I meets the standards for spraying pattern test, skin moisture and % inhibition highest in the antioxidant test. The preparation face mist most optimalis formula I with a ratio of pomegranate peel extract and mangosteen peel (10:15).

Kenikir Leaf Extract (Cosmos Caudatus Kunth) is a medicinal plant that can be used to heal cut wounds. This research aims to determine the compound content in kenikir leaves, determine the physical quality and stability of kenikir leaf extract ointment preparations, and determine the effective concentration for healing cuts on the backs of male New Zealand White rabbits. This type of research is experimental, making ointment formulations using kenikir leaf extract made in 3 concentrations, namely 10%, 15% and 20%. In ointment testing, organoleptic tests, homogeneity tests, spreadability tests, adhesion tests, pH and viscosity tests are carried out. Testing the effectiveness of wound healing using 5 groups, namely Betadine ointment (+), ointment base (-), kenikir leaf extract ointment 10%, 15% and 20% on 5 rabbits with a wound length of 1.5 cm was carried out until the 3rd day. 7. The data was processed statistically using One Way ANOVA (Analysis of Variant) and continued with the LSD (Least Significant Difference) test. The results of this research show that kenikir leaf extract ointment 10%, 15% and 20% produces an ointment that meets the requirements of the organoleptic test, homogeneity test, spreadability test, adhesiveness test and pH test, as well as providing healing effects on cuts and wounds in rabbits and The most effective healing power is 20% kenikir leaf extract ointment.

Introduction: Lately, the use of natural cosmetics can have a good effect on the skin. One of them is traditional beauty treatments. The Serum is a preparation with low viscosity, because of its low viscosity serum is categorized as an emulsion preparation. From the results of research that has been carried out, ethanol extract of Hati Tanah tuber has great potential as an anti-acne because it can inhibit the growth of acne-causing bacteria such as Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acnes. With the innovation of serum ethanol extract of Hati Tanah tuber, it can be an option for the community later in the treatment of acne. Methods: The formulation is made in 4 (four) formulas with different concentrations of the main ingredients used. Next is to weigh all the ingredients needed according to each formula. Then physical properties test performed. a) Organoleptic test; b) Homogeneity Test; c) pH test; d) Spreadability Test and e) Adhesion Test. Results: The organoleptic test was carried out directly by looking at the shape and color and smelling the serum preparation of the ethanol extract of Tanah Hari tubers. The test results show that at F0 (0%) the color is clear, for F1 (0,5%) it is quite pale pink, F2 (1%) is bright pink and F3 (5%) is dark red. For scent organoleptic, F0 indicates no scent, for F1 and F2 there is a distinctive scent from the Hati Tanah tuber which is slightly pungent, while F3 shows a pungent aroma typical of the Hati Tanah tuber. The resulting serum has a thick texture at F0 and F1, a watery texture at F2 and a thinner texture at F3. All of the formulas have met the physical test requirement of organoleptic properties, homogeneity, pH, and adhesion test. Conclusion: The simplicia of Hati Tanah tuber can be made into an innovative cosmetic preparation in the form of serum with a concentration of 0,5%, 1% and 5%. From the results of testing the physical properties of the formulations, all of the formulas have met the physical test requirement of organoleptic properties, homogeneity, pH, and adhesion test.

Antioxidants can be given to the skin through cosmetics such as face toners. One source of antioxidants is obtained through the fermentation of butterfly pea flower kombucha (Clitoria ternatea L.), which has been proven to have better antioxidant activity than butterfly pea flower extract alone. This study aimed to determine the metabolite compounds in butterfly pea flower kombucha and create a facial toner formulation with good antioxidant activity. The butterfly pea flower kombucha was fermented for 6 days at room temperature. Facial toners were made with concentrations of 5%, 7,5% and 10%. Evaluation of the face toner includes organoleptic, homogeneity, pH, specific gravity, and viscosity tests. The antioxidant activity test used the DPPH (1,1-diphenyl-2-pycrilhydrazil) method, with vitamin C as a positive control. The results showed that there were alkaloids, flavonoids, saponins, and terpenoid compounds in butterfly pea flower kombucha. Face toners with concentrations of 5%, 7.5%, and 10% could be prepared and met all evaluations (organoleptic tests, homogeneity, pH, specific gravity, and viscosity). Antioxidant test results on formula 1 IC50 50,21±SD 0,01 (strong), formula 2 IC50 44,32±SD 0,02 (very strong) and formula 3 IC50 38,62±SD 0,03 (very strong). The higher the concentration of butterfly pea flower kombucha, the higher the antioxidant activity. Butterfly pea flower kombucha (Clitoria ternatea L.) which is formulated as a face toner preparation has strong to very strong antioxidants, and the increasing concentration of active ingredients had an effect on antioxidant activity. Keywords: face toner, butterfly pea flower kombucha, DPPH method, antioxidant activity

Mangrove (Rhizophora mucronata) contains flavonoids, saponins and tannins. Compounds contained in mangrove leaves have antiinflammatory, anti viral and antimicrobial activites. Based on the compound, mangrove (Rhizophora mucronata) can be used an ointment for cuts. This study aims to test the preparation ofmangrove leaf ethanol extract ointment on the healing of cuts in mice. The process of extracting mangrove leaves (Rhizophora mucronata) uses the maceration method. In testing the ointment preparations, organoleptic tests, homogeneity tests, pH tests, spreadability tests and viscosity tests were carried out. Testing the effectiveness of wound healing using 5 groups consisting of 3 mice. Each group was giffen different treatment, namale K- was given an ointment base, K+ was given betadine ointment, F1 was given an ointment with a concetration of 2,5%, F2 was given an ointment with a concentration of 5% and F3 was given an ointment with a concentration of 7,5%. The results showed that the ointment met the standard organoleptic test requirements, test homogeneity, pH test, spreadability test and viscosity test. Based on the results of the incision test on mice, the most effective ointment preparation was a preparation with a concentration of 5%

Blush is a type of decorative cosmetic used to give red color in the cheeks area so that the face looks prettier, fresh and dimensional. Currently there are many blush on in the market that contain hazardous chemicals. Then a blush on made from Rosella flower extract (Hibiscus sabdariffa) containing anthocyanin pigments uses as a safer dye alternative. This blush on cream formula made using Rosella flower extract with concentrations of 1%, 3%, and 5% with the resulting color of pale pink, pink and purplish pink. Evaluations test undertaken include organoleptic test, homogeneity test, spreadability test, pH test, viscosity test and stability test. The results obtained based on organoleptic test, homogeneity test, spreadability test, pH test, viscosity test shows that all three formulas entered into the required range of values.

Background: Acne vulgaris is a chronic inflammatory skin disease with a complex pathogenesis, involving sebaceous glands, follicular hyperkeratosis, bacterial overgrowth, immune and inflammatory responses. Objective: This formulate the ethanol extract of banana peel and pineapple peel into cream preparations and to test the effective antibacterial activity of the combination of banana peel and pineapple peel ethanol extract cream against Propionibacterium acnes. Methods: This research was conducted experimentally with maceration extraction using 70% ethanol as solvent. Acne cream is made with a concentration ratio between (Banana peel: Pineapple peel) namely F1 (Cream Base), F2 (5%:15%), F3 (7.5%:12.5%), F4 (10%:10% ), F5 (12.5%:7.5%), F6 (15%;5%), F7 (20%:0%) and F8 (0%;20%). Then, the physical stability test was carried out in the form of organoleptic test, viscosity test, pH test, homogeneity test, spreadability test and cream type test. Followed by testing the bacteria Propionibacterium acnes in Na media. Results: The antibacterial activity test on F3 showed that the diameter of 20.18 mm was relatively larger than F1 of 0mm, F2 of 18.63 mm, F4 of 18.38 mm, F5 of 16.48 mm, F6 of 18.28 mm, F7 of 13.91 mm and The F8 is 16.11 mm. Conclusion: Cream preparations from formula 1 to formula 8 the cream stability organoleptic test, viscosity test, pH test, homogeneity test, test spreadability and cream type test. The cream preparation in formula 3 has the widest diameter for antibacterial activity compared to other formulas.

Unhealthy lifestyles and air pollution can cause the number of free radicals in the body to increas. The formation of free radicals is a widely accepted important mechanism that causes skin aging. For this reason, the body needs antioxidants that can neutralize very dangerous free radicals. A plant whose efficacy as an antioxidant has been proven is the pagoda flower plant. Another plant used to ward off free radicals is the rhizome of Temu Kunci (Boesenbergia rotunda (L.) Mansf.) which is from the Zingiberaceae family which has compounds that have the potential to act as antioxidants. This research aims to formulate a lotion preparation of pagoda flower extract and ginger root rhizome extract as well as carrying out a stability test of the preparation and determining the IC 50 value using the DPPH method. The research used the maceration method with 96% ethanol. Then the extract is formulated into a lotion with varying concentrations of pagoda flower extract: ginger rhizome extract, namely: F1(1:1); F2(2:1); F3(1:2). The preparations of pagoda flower extract lotion and ginger root rhizome extract were eval_uated for organoleptic tests, pH tests, homogeneity tests, viscosity tests and hedonic tests and antioxidant tests were carried out with a wavelength of 516nm. The results of the research on pagoda flower extract lotion and ginger root rhizome extract have met the requirements for organoleptic tests, pH test, homogeneity test, and viscosity test. The IC50 value of the lotion preparation of ethanol extract of pagoda flowers and ethanol extract of ginger rhizome showed a strong category (50-100) with an IC50 value of F1 86.13 µg/ml, F2 73.91 µg/ml and F3 75.83 µg/ml.

Antioxidant compounds are compounds that counteract or reduce the negative impact of oxidants, which work by donating one electron charge so that the activity of these oxidant compounds can be prevented or inhibited. The aim of this study was to determine the formula for cream variation of extract from banana peel which has the best antioxidant activity with good quality and stability of the preparation. In this study, the antioxidant cream used 3 (three) series of concentrations of the methanol extract of the skin of the plantain (Musa paradisiaca L.) fruit, namely 0.5%, 1% and 2%. The methanol extract of plantain peel was obtained by maceration method with methanol as solvent. Antioxidant cream was tested for physical quality and stability which included organoleptic test, viscosity test, pH test, homogeneity test, adhesion test, and spreadability test. The antioxidant power test was carried out using the DPPH (1,1-Diphenyl-2-Picrylhydrazyl) method using a UV-Vis spectrophotometer with a wavelength of 400-600 nm. The results of the study were further analyzed statistically using the Tukey method and one-way ANOVA. The results showed that the methanol extract of plantain peel had very strong antioxidant activity, and cream preparations could be made with various extract concentrations and the IC50 values of formula 1, formula 2 and formula 3 were 53.81 ppm, 53.29 ppm and 53.21 ppm.

Catfish (Clarias sp) contains albumin which is an important protein needed by the human body, especially in the process of healing diseases. Giving this catfish extract functions as a nutrient, it is hoped that this nutrition can improve nutrition, increase the immune system and speed up the wound healing process or post surgery. This research is an experimental research, in which the albumin ointment formulation of catfish (clarias sp) extract was carried out, with variations of vaselin flavum and adeps lanae ointment bases in three formulations namely formulation 1, formulation 2 and formulation 3. The results of catfish extract (clarias sp) were then formulated in the form of an ointment. Then an evaluation of physical properties was carried out including organoleptic evaluation, homogeneity test, spreadability test, adhesion test, pH test. The results showed that the albumin extract ointment for catfish (Clarias sp) with variations in ointment base made a difference to the evaluation of albumin extract for catfish (Clarias sp) which included organoleptic tests (soft shape, white to yellow), homogeneity test (has round particles, irregular, no foreign particles), spreadability test (FI, 5.16cm: FII, 5.23cm: FIII, 5.06cm), adhesion test (FI, 3.19 seconds, FII 2.27 seconds, FIII 3.24 seconds), pH test (FI 6, FII 6, FIII 6). The conclusion that the vaseline flavum ointment combined with adeps lanae from catfish albumin extract meets the quality consists of an evaluation. homogeneity test, organoleptic test, adhesion test, spreadability test and pH test.