Formula omitted] deconvolution of fluorescence decay data

Abstract

An approach for the deconvolution of multiexponential fluorescence decay data in which a single exponential decay is used in place of the usual excitation profile is described. For analysis by the method of moments, the resulting decay lifetimes are identical to those in the multiexponential decay, while the preexponential factors are a simple function of the true values and the parameters of the single exponential decay. This approach, which we call the FF deconvolution method, is capable of eliminating the errors in decay analyses which arise from the wavelength dependence of the instrument response function.