Abstract

E107 is a mutant of pea (Pisum sativum L. cv. Sparkle) characterized by necrotic spots on its leaves, high Fe accumulation in its shoot, and decreased nodulation. Fresh, cleared, and fixed tissues of the mutant and its parent were observed by light and electron microscopy. E107 has cell wall appositions in its basal leaflets and in the endodermis of its lateral roots. The wall deposits in the leaves are layered and contain callose and phenols. The appositional walls in the lateral root endodermis, made of lignin or of phenols, are not organised. We hypothesize that the appositions are stress responses to metal toxicity. Key words: pea mutant, wall appositions, histochemistry, iron toxicity.

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