Abstract

The national infrastructure FoodOmicsGR_RI coordinates research efforts from eight Greek Universities and Research Centers in a network aiming to support research and development (R&D) in the agri-food sector. The goals of FoodOmicsGR_RI are the comprehensive in-depth characterization of foods using cutting-edge omics technologies and the support of dietary/nutrition studies. The network combines strong omics expertise with expert field/application scientists (food/nutrition sciences, plant protection/plant growth, animal husbandry, apiculture and 10 other fields). Human resources involve more than 60 staff scientists and more than 30 recruits. State-of-the-art technologies and instrumentation is available for the comprehensive mapping of the food composition and available genetic resources, the assessment of the distinct value of foods, and the effect of nutritional intervention on the metabolic profile of biological samples of consumers and animal models. The consortium has the know-how and expertise that covers the breadth of the Greek agri-food sector. Metabolomics teams have developed and implemented a variety of methods for profiling and quantitative analysis. The implementation plan includes the following research axes: development of a detailed database of Greek food constituents; exploitation of “omics” technologies to assess domestic agricultural biodiversity aiding authenticity-traceability control/certification of geographical/genetic origin; highlighting unique characteristics of Greek products with an emphasis on quality, sustainability and food safety; assessment of diet’s effect on health and well-being; creating added value from agri-food waste. FoodOmicsGR_RI develops new tools to evaluate the nutritional value of Greek foods, study the role of traditional foods and Greek functional foods in the prevention of chronic diseases and support health claims of Greek traditional products. FoodOmicsGR_RI provides access to state-of-the-art facilities, unique, well-characterised sample sets, obtained from precision/experimental farming/breeding (milk, honey, meat, olive oil and so forth) along with more than 20 complementary scientific disciplines. FoodOmicsGR_RI is open for collaboration with national and international stakeholders.

Highlights

  • In recent years, there has been an increased awareness towards the evaluation of food quality and safety in relation to human health and life

  • It includes the General Assembly (GA) that is composed of delegates from all involved institutions and a Steering Committee of five members from the participating institutions that manage the operation of the RI

  • The aggregate capability for capital instrumentation of the laboratories reaches five hybrid time of flight mass spectrometers Q-TOF-MS hyphenated to UPLC systems (two with ion mobility capability(TIMS-TOF-MS)), a UPLC-QTRAP 6500+ MS/MS, more than 10 triple quadrupole MS/MS instruments hyphenated to UPLC, four UPLC-Orbitrap High-Resolution Mass Spectrometry (HRMS), three HPLC-MS (High-performance liquid chromatography mass spectrometry) single quadrupole instruments, an SFC-UV/MS system, more than 8 GC-MS (Single quadrupole analysers), and three GC-MS/MS (Gas chromatography–mass spectrometry systems with triple quadrupole MS)

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Summary

Introduction

There has been an increased awareness towards the evaluation of food quality and safety in relation to human health and life. Foodomics [1] combines food/nutrition sciences with advanced analytical techniques and bioinformatics, applying a hypothesis-free approach to globally map the composition of foods or biological fluids of food consumers, to elucidate critical questions, and address new challenges of a globalized world These may include: safety/security, sustainability, authentication, traceability, improvement of food production in response to environmental changes, the potential use of food waste and other fields. This holistic perspective requires investments in research expertise and instrumental facilities, but in return it provides big data, which after the application of advanced statistical analysis and bioinformatics can generate new knowledge, highlighting unlooked-for patterns and associations of biomolecules. It is important to mention that the long-lasting collaborations with a large number of researchers from the private sector (food producers, clinics, CRO laboratories, life science companies and analytical instrument/technology vendors), as well as international research organisations and different regulatory bodies and authorities (Eurachem, NIST, mQACC, Norman network, IRMM JRC and other national and international bodies)

Governance
Access to Facility
Advancing Analytical Capability of the RI
Advancing and Harmonising the Analytical Portfolio
MS Based-Metabolomics
NMR Based-Metabolomics
Genetic and Genomic Analyses for Traceability of Animal Organisms
Proteomics Analysis
Elemental Metabolomics for Food Authentication
Sample Banking
LC-MS and GC-MS Technology
MALDI-TOF-MS Technology
Elemental Metabolomics Technology
Data Combination
Valorization of Agro-Industry by-Products
Assessment of Food Safety
Impact on the National Research Environment
Findings
Concluding Remarks

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