Summary The provision of an adequate food supply (bacteria) after freezing and thawing the rotifer Philodina is shown to increase the percentage survival. Furthermore, variations in the survival statistics as a function of cooling rate appear to be minimized by the addition of food after thawing. Both glyceerol and DMSO provide high levels of cryoprotection, DMSO being slightly more effective. Recovery of close to 100% of a population can be achieved with proper attention to pretreatment, cooling, and thawing procedures.

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