Abstract
The simple fluorometric method for determination of added pyridoxine-HCl in enriched food, based on oxidation of pyridoxine to 4-pyridoxic acid by means of KMnO4, has been modified for determination of total B6 in soya bean. In comparison to the other fluorometric procedures, the proposed method is the simplest one, rapid and easy to perform, demanding the least time for oxidation and not using hazardous chemicals (KMnO4 instead of KCN). Good recovery, low relative standard deviation and low total error allow the proposed procedure to be included into the group of excellent methods.
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