Abstract
A highly sensitive method has been developed to measure quantitatively the DNA content of yeast cells. The reaction between the fluorescent dye 4′, 6-diamidino-2-phenyl-indol-dihydrochloride and DNA in a crude lysate from protoplasts can be measured without prior extraction of the DNA. Interferences by the high protein and RNA content of yeast cells, which often limit the accuracy of common methods employed in the determination of DNA content, were eliminated by suitable enzymatic pretreatment of the cell lysate.
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