Abstract

A sensitive, specific fluorometric method has been combined with microdiffusion to measure cyanide in biological fluids. Cyanide reacts with p-benzoquinone to form fluorescent product(s) which are determined in dimethyl sulfoxide. Approximately 0.001 μmol/ml cyanide can be analyzed by this procedure. Sodium thiosulfate interferes with the determination of cyanide by the standard colorimetric procedure, but not with this fluorometric method. Blood cyanide concentrations in mice pretreated with sodium thiosulfate were compared by the fluorometric and colorimetric methods. These results demonstrate that thiosulfate interferes with the detection of cyanide colorimetrically, but not fluorometrically.

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