Abstract
In this present study the bioconjugated fluorescent silica nanoparticles give an efficient fluorescent-based immunoassay for the detection of pathogen. The synthesized silica nanoparticles were polydispersed and the size of the silica nanoparticles was in the range of 114–164 nm. The energy dispersive X-ray spectrophotometer showed the presence of silica at 1.8 kev and the selected area diffractometer showed amorphous nature of silica nanoparticles. The FTIR spectrum confirmed the attachment of dye and carboxyl group onto the silica nanoparticles surface. The fluorescent silica nanoparticles showed highly efficient fluorescence and the fluorescent emission of silica nanoparticles occurred at 536 nm. The SEM image showed the aggregation of nanoparticles and bacteria. The growth of the pathogenicE. coliwas controlled using silica nanoparticles; therefore silica nanoparticles could be used in food packaging material, biomedical material, and so forth. This work provides a rapid, simple, and accurate method for the detection of pathogen using fluorescent-based immunoassay.
Highlights
The fundamental building blocks of nanotechnology are nanoparticles [1]
Due to the hydrolysis process, the ethoxy groups are replaced by TEOS; the hydrolysis reaction initiates by the attachment of hydroxyl anions on TEOS molecules [6, 7]
The Si–O–Si bridges are formed by the alcohol condensation of hydroxyl group of intermediate reaction with ethoxy group of other TEOS, or the water condensation of hydroxyl group of another intermediate by hydrolysis reaction [7, 8]
Summary
The fundamental building blocks of nanotechnology are nanoparticles [1]. The particulate dispersions or solid particles with a size in the range of 10–1000 nm are called nanoparticles [2]. Silica is encompassed of a honeycomb-like porous structure with hundreds of empty channels Due to their unique properties such as high surface areas, large pore volumes, tuneable pore sizes with a narrow distribution, and tuneable particle diameters, currently silica nanoparticles have been intensively investigated in materials research [3]. The cohydrolysis process is carried out by mixing suitable amount of organosilane with TEOS which facilitates particles conjugation and particle dispersion; various functional groups such as thiol, amine, carboxyl groups, and phosphonate groups could be attached by using this method [15, 16] In this present work, silica nanoparticles surface is modified with carboxyl group with the help of silane coupling agent EDC.
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