Abstract

A fluorescent probe for Cu(II) ion is presented. It is based on the rhodamine fluorophore and exhibits high selectivity and sensitivity for Cu(II) ion in aqueous methanol (2:8, v/v) at pH 7.0. The response is based on a ring opening reaction and formation of a strongly fluorescent 1:1 complex. The response is reversible and linear in the range between 50 nM and 900 nM, with a detection limit of 7.0 nM. The probe was successfully applied to fluorescent imaging of Cu(II) ions in HeLa cells.

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