Abstract
The ineraction between riboflavin (RBF) and tryptophan (Trp) was investigated using fluorescence spectroscopy and UV-vis absorption spectroscopy under physiological conditions. The fluorescence of Trp was quenched by RBF via dynamic quenching, which was analyzed using the Stern-Volmer relation. The value of the Forster distance R0 (2.31 nm) was obtained according to the Forster's theory of nonradiative energy transfer. Under physiological conditions, a linear relationship could be established between the quenched fluorescence intensity of Trp and the concentration of RBF in the range of 5.8 × 10(-7) -2.0 × 10(-5) mol/L. The detection limit was 1.8 × 10(-7) mol/L. The method was successfully applied to determine riboflavin concentrations in pharmaceutical samples.
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