Fluorescence polarisation immunoassays for rapid, accurate and sensitive determination of mycotoxins

Abstract

Fluorescence polarisation immunoassay (FPIA) is a type of homogeneous assay. For low molecular weight antigens, such as mycotoxins, it is based on the competition between an unlabeled antigen and its fluorescent-labelled derivative (tracer) for an antigen-specific antibody. The antigen content is determined by measuring the reduction of fluorescence polarisation signal, which in turn is determined by the reduction of tracer molecules able to bind antibody in solution. To develop a competitive FPIA for mycotoxin measurement the tracer has to be synthesised and its binding response with a specific antibody should be tested. Selectivity and sensitivity of the FPIA methods are strictly related to the antibody/tracer combination used. Several FPIA methods for the detection of the major mycotoxins, including aflatoxins, fumonisins, ochratoxin A, deoxynivalenol, T-2 and HT-2 toxins and zearalenone in food and beverages have been developed in the last decade. Basic principles, key elements, advantages and limitations of these methods are reviewed. These FPIA methods are simple, readily automated, rapid, and suitable for high-throughput screening, as well as for the reliable quantitative determination of mycotoxins in foods and commodities.