Abstract
A highly sensitive fluorescence high-performance liquid chromatography, based on the fluorescence of thiochrome which is formed from oxidation of thiamine by the molybdoheteropoly acid of phosphate has been developed for the determination of trace microamounts of phosphate. The method permits the determination of phosphate in the range of 0.1 to 1.0 ng as P/10 Μl sample solution (3.23×10−7 to 3.23×10−6 mol l−3 as P or PO4), and it may be possible to apply the proposed method to the microanalysis of phosphate in various biological samples.
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