Abstract
Summary This study was carried out to clarify the fluctuation of the ploidy level in the epidermis and compare the level of polyploidy with related evidence during the penultimate and ultimate larval instar of Bombyx mori. Flow cytometry analysis revealed that the percentage of 8C and 4C cells decreased with the increase of 16C cells in the fourth larval instar, while 32C cells appeared for the first time on day 3 in the fourth larval instar. At the beginning of the fifth larval instar, most of cells were 16C or 32C, and the percentage of 16C cells was twice that of 32C cells. The percentage of 16C and 32C cells remained almost constant during the feeding stage, followed by a decrease during the spinning stage. Changes of ploidy level, DNA synthesis, and mitosis were observed when the concentration of hemolymph ecdysteroid was high in both the penultimate and ultimate larval instar. Mitosis was induced in the presence of 20E. The present results indicate that ecdysteroid affects the DNA synthesis, mitosis, and polyploidy in the epidermis in the penultimate and ultimate instar of Bombyx mori.
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