Abstract
For analysis of the promoter of a gene for chalcone synthase ( GTCHS1) from Gentiana triflora, a cDNA was cloned for chalcone synthase (CHS) from a cDNA library of petals of Gentiana. Using the sequence of GTCHS1, the promoter region of GTCHS1 was cloned by the inverse polymerase chain reaction. The promoter was fused to a gene for β-glucuronidase (GUS) and the construct was introduced into Petunia hybrida. Measurements of the GUS activities of transformants indicated that the GTCHS1 promoter strongly directed the expression of GUS in flower limbs, while the 35S promoter of cauliflower mosaic virus (CaMV) directed expression of the reporter gene in all tissues. Histochemical staining of GUS activity revealed that the GTCHS1 promoter strongly directed the expression of GUS in the inner epidermis, at sites where most of the anthocyanin accumulated. The sequence of the GTCHS1 promoter included a consensus sequence of the MYB protein-binding site, five consensus sequences of the MYC protein-binding site, one core sequence of a G-box and three P-box-like sequences.
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