Abstract
Glutamate dehydrogenase is immobilized on poly(vinyl alcohol) beads and packed into a stainless-steel column (3 cm × 4 mm i.d.). Serum is deproteinized with tungstic acid. Sample solution (30 μl) is injected into the carrier stream [5 mM NAD + in glycine buffer (pH 9.5)]. The NADH formed is detected at 465 nm (excitation at 340 nm). The calibration graph is linear for 0.5–500 μM glutamate; the detection limit is 0.2 μM.
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