STAR protocols | VOL. 3

Flow cytometry analysis of endothelial cells and subsets of exhausted CD8+ Tcells in murine tumor models.

Publication Date Jun 17, 2022


Here, we present a protocol for flow cytometry analysis of endothelial cells (ECs) and CD8+ Tcells in murine tumor models, at baseline and after cancer immunotherapy with anti-PD-1/anti-CTLA-4 antibodies. We provide gating strategies for identification of specific cell subsets including ECs from tumor-associated high endothelial venules (TA-HEVs), stem-like, and terminally exhausted CD8+ Tcells. This protocol represents a valuable tool for the analysis of rare subsets of tumor ECs and CD8+ Tcells with critical roles in antitumor immunity. For complete details on the use and execution of this protocol, please refer to Asrir etal. (2022).


Subsets Of Exhausted CD8 Murine Tumor Models Endothelial Cells Subsets Of CD8 Exhausted CD8 Analysis Of Subsets Antitumor Immunity Cancer Immunotherapy Murine Models Identification Of Subsets

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No potential conflict of interest was reported by the authors. The conception and design of the study, acquisition of data, analysis and interpretatio...

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