Abstract
The development of the testis of the cricket Teleogryllus commodus has been studied using flow cytometry. From a laboratory-reared population of insects, total testis tissue was prepared at approx. 48-h intervals for the entire development period extending from the fifth instar (28 days after eclosion) to the imaginal moult (about 60 days after eclosion). Cells were stained with DNA-specific fluorochromes and the temporal sequence of the development of the testis as well as the timing of specific meiotic events quantitatively determined. Testis development studied in this fashion is seen as changes in the relative frequency of cells with 1, 2, and 4C DNA content. Pre-spermiogenic processes of spermatogenesis (change in the frequency of cells with 2 and 4C DNA content) occur continuously during the fifth-eighth instars, while the appearance of 1C cells (initiation of spermiogenesis) occurs abruptly at the transition between the sixth and seventh instars.
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