Abstract
By a screen designed to isolate new fission yeast genes required for chromosome segregation, we have identified mal2+. The conditionally lethal mal2-1 allele gives rise to increased loss of a nonessential minichromosome at the permissive temperature and leads to severe missegregation of the chromosomes at the nonpermissive temperature. Cloning by complementation and subsequent sequence analysis revealed that mal2 is a novel protein with a mass of 34 kDa. Cells containing a mal2 null allele were inviable, indicating that mal2+ is an essential gene. Fusion of mal2 protein to the green fluorescent protein (GFP) showed that mal2 was predominantly localized in the nucleus. Sensitivity to microtubule-destabilizing drugs and strong genetic interactions with alpha1-tubulin suggest an interaction of the mal2 protein with the microtubule system. Spindle formation and elongation were not detectably affected in the mal2-1 mutant as determined by indirect immunofluorescence. However, anomalous chromosome movement on the spindle leading to aberrant distribution of the chromosomal material was observed.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
