Abstract
In this study, we first described the complete mitochondrial genome for the red crab (Charybdis feriata), elucidated its phylogenetic relationship among 20 species within Decapoda, and estimated the population genetic diversity. The mitochondrial genome was 15,660 bp in size and encoded 13 protein-coding genes, 22 transfer RNA (tRNA) genes, and two ribosomal RNA genes. The gene arrangement of the mitochondrial genome was the same as that of its sister species, C. japonica. Phylogenomic analysis suggested that genus Charybdis should be classified into subfamily Portuninae but not into subfamily Thalamitinae. Moreover, a total of 33 haplotypes of complete cytochrome c oxidase subunit I gene were defined in 70 individuals of C. feriata derived from three localities. Haplotype diversity and nucleotide diversity values among three localities indicated a high level of genetic diversity in C. feriata. AMOVA analysis suggested a low level of genetic differentiation among the three localities (FST = 0.0023, P > 0.05). Neutrality tests and mismatch analysis revealed that C. feriata might have undergone a population expansion event that possibly occurred in the last 61,498 to 43,814 years. This study should be helpful to better understand the evolutionary status, and population genetic diversity of C. feriata and related species.
Highlights
Microsatellite markers, respectively[12,13]
The lack of complete mitochondrial genome has limited the development of population genetic diversity and molecular evolution for this species
The purpose of this study is to report the complete mitochondrial genome for C. feriata, elucidate its evolutionary status, and estimate population genetic diversity and differentiation
Summary
Microsatellite markers, respectively[12,13]. To estimate and conserve this important crab resource, genetic studies such as population genetic diversity and evolutionary history should be carried out. The phylogenetic analysis based on 13 protein-coding genes from the mitochondrial genome[21] supported the former opinion. It is thought to be an ideal marker for studies on population genetic diversity, molecular phylogeny, and species identification because of its high mutation rate, simple structure, abundant distribution, and maternal inheritance[22,23,24]. The lack of complete mitochondrial genome has limited the development of population genetic diversity and molecular evolution for this species. The purpose of this study is to report the complete mitochondrial genome for C. feriata, elucidate its evolutionary status, and estimate population genetic diversity and differentiation. This work should be helpful to better understand the evolutionary status and population genetic diversity of C. feriata and other related crustacean species
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
