Abstract
BackgroundThe NDM-1 carbapenemase has been identified in 2008 in Enterobacteriaceae. Since then, several reports have emphasized its rapid dissemination throughout the world. The spread of NDM carbapenemases involve several bla NDM gene variants associated with various plasmids among several Gram negative species.MethodologyA multidrug-resistant E. coli isolate recovered from urine of a patient who had travelled to Burma has been characterized genetically and biochemically.Principal Findings E. coli COU was resistant to all antibiotics tested except amikacin, tigecycline, fosfomycin, and chloramphenicol. Analysis of the antibiotic resistance traits identified a metallo-ß-lactamase, a novel NDM variant, NDM-7. It differs from NDM-4 by a single amino acid substitution sharing an identical extended spectrum profile towards carbapenems. The bla NDM-7 gene was located on an untypeable conjugative plasmid and associated with a close genetic background similar to those described among the bla NDM-1 genes. The isolate also harbours bla CTXM-15 and bla OXA-1 genes and belonged to ST167.SignificanceThis study highlights that spread of NDM producers correspond to spread of multiple bla NDM genes and clones and therefore will be difficult to control.
Highlights
First identified in K. pneumoniae and E. coli isolates recovered from a patient previously hospitalized in India, NDM carbapenamase producers are of great concern worldwide [1]
A carbapenem-resistant E. coli COU isolate was recovered from the patient urine culture
Antibiogram determined by the disc diffusion technique and interpreted according to guidelines of the Clinical and Laboratory Standards Institute [18], revealed that E. coli COU was resistant to penicillins, expanded-spectrum cephalosporins, and carbapenems
Summary
First identified in K. pneumoniae and E. coli isolates recovered from a patient previously hospitalized in India, NDM carbapenamase producers are of great concern worldwide [1]. Analysis of the antibiotic resistance traits identified a metallo-ß-lactamase, a novel NDM variant, NDM-7. NDM-4, NDM-5 and NDM-6 have been described from E. coli isolates from patients with history of hospitalization in India [8,9,10].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
