Fine structure of secretory ameloblasts in kitten tooth germs, with special regard to intercellular junctions as revealed by freeze-fracture

Abstract

Using both thin sectioning and freeze-fracture replication, junctional complexes at both proximal and distal ends of the cells consisted of tight junctions in close association with gap junctions and desmosomes. The tight junctions generally consisted of smooth, continuous rows of particles on the P-face and corresponding patterns of shallow grooves or. the E-face of cell membranes. Though sealing of paracellular spaces around the ameloblasts in the proximal junctional complex was incomplete, there was complete sealing around the ameloblasts and well-developed meshwork structures of tight junctions in distal junctional complexes. Discontinuous and free-ending strands of tight junctions were frequent in junctional complexes, suggesting that ameloblast distal junctional complexes serve, not only as a barrier to high molecular passive substances through the ameloblast layer, but also as a channel for ions and low-molecular substances. Ameloblasts were firmly connected with stratum intermedium cells by desmosomes and gap junctions. The gap junctions on ameloblast basal and lateral surfaces probably function in intercellular transfer of ions and low-molecular substances between the stratum intermedium and ameloblasts and in control of ameloblast cytodifferentiation.