Abstract
Mycoplasma bovis in cattle is difficult to diagnose. Recently, the ID screen® mycoplasma bovis indirect ELISA (ID screen) was commercially released by IDVet. The objectives of this study were to: (1) gain and share experience of using the ID screen in adult dairy cows under field conditions; (2) determine the correlation between antibody levels in milk and serum and (3) compare the ID screen results with those of the Bio K 302 (BioX 302) ELISA from BioX Diagnostics. Paired serum and milk samples were collected from 270 cows from 12 Danish dairy herds with three categories of M. bovis disease history. The ID screen tested nearly all cows positive in all, but the three non-infected herds, while the BioX 302 tested very few cows positive. The ID screen is therefore a much more sensitive test than the BioX 302. However, cows in five exposed herds without signs of ongoing infection and two herds with no history of M. bovis infection also tested ID screen positive. Therefore, the performance and interpretation of the test must be investigated under field conditions in best practice test evaluation setups. A concordance correlation coefficient of 0.66 (95% CI: 0.59–0.72) between the ID screen serum and milk results indicates that milk samples can replace serum samples for the ID screen diagnosis of M. bovis in adult cows.
Highlights
Mycoplasma bovis (M. bovis) is an emerging bacterium associated with disease in cattle of all ages in many countries around the world [1]
Between the ID screen serum and milk results indicates that milk samples can replace serum samples for the ID screen diagnosis of M. bovis in adult cows
The fact that M. bovis bacteria lead to so many different disease manifestations and varying test responses in different age groups, and the fact that there is not one single diagnostic material that can test for and differentiate between all these disease manifestations makes it difficult to diagnose M. bovis-associated disease [4]
Summary
Mycoplasma bovis (M. bovis) is an emerging bacterium associated with disease in cattle of all ages in many countries around the world [1]. Diagnosing M. bovis is challenging at both animal and herd level. M. bovis-associated disease can be diagnosed by using bacterial culture or PCR on body fluids or organ specimens and antibody measurements in serum or milk [3]. The fact that M. bovis bacteria lead to so many different disease manifestations and varying test responses in different age groups, and the fact that there is not one single diagnostic material that can test for and differentiate between all these disease manifestations makes it difficult to diagnose M. bovis-associated disease [4]. The first and previously only commercially available test for antibodies directed against M. bovis was produced by BioX Diagnostics in Belgium. The Bio K 302 ELISA kit (BioX 302) has been reported to have low sensitivity ranging from 0.37–0.50 and specificity ranging
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