Abstract
The highest feeding rates were obtained with 4- to 7-day-old mosquitoes that remained in the feeding chambers 3 to 4 hours and fed in an ambient temperature of 25° C., through Silverlight membranes. The outdated human blood was held at 4° C. until needed, then warmed to the desired temperature just prior to its use. Somewhat lower feeding percentages obtained in several experiments were probably due to use of mosquitoes that were too young, or to the presence of males and/or excessive numbers of females in the feeding chambers. Mosquitoes deprived of sugar for 48 hours and of water for 24 hours before the blood meal fed better and ingested more blood than mosquitoes that were allowed sugar and water until just prior to membrane feeding. Partially fed mosquitoes ingested from 0.29 to 0.75 mg. of blood, fully engorged ones 1.12 to 2.44 mg., when fed through Silverlight, Nearkid, Zephir, and Fourex membranes.
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