Abstract
CURRENTLY two methods are available for measuring the color deposited in poultry or poultry products from feed pigments. The chick assay measures the pigmentation in the shanks, and the hen assay measures the pigmentation in the yolks of eggs. The chick assay is the least desirable of the two for screening purposes since fat-soluble pigments exist that are not deposited in the skin but will color egg yolks (Fritz et al., 1958). A more desirable assay would depend upon the deposition of color in egg yolks, thereby reducing the possibility of missing an effective pigment intended for this purpose. The hen also appears to be a more sensitive assay animal than the chick. Fritz et al. (1958) found that at least 25 mg. of xanthophyll per pound of feed was required to obtain a dark colored egg yolk, whereas only 10 mg. was needed to give normal pigmentation in broilers… .
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