FEATURES OF RESTRUCTURING OF THE KIDNEY AND HEART DURING ALLOXAN HYPERGLYCEMIA

Abstract

The progressive increase in the number of diabetics leads to a detailed study of its course in experimental animal models. Objective: To study the morphological reconstruction of kidney and heart in rats model alloxan hyperglycemia. Materials and methods. 12 male laboratory rats aged 7 months were divided into two groups: control (6 animals) and experimental (6 animals). The experimental animals were injected alloxan once intraperitoneally at 40 mg/kg. Blood glucose was measured 2, 12 and 24 hours after injection and then glucose level was checked every week. The average glucose level remained 11 mmol / L ± 2 mmol / L. The animals were taken out of the experiment on the 21th and the 45th day by decapitation under ether anesthesia. Histological preparations of the heart and kidney were stained with hematoxylin&eosin. Results. On the 21st day of the experiment there are hemorrhages in the kidney, uneven blood supply vessels and perivascular edema in the myocardium. On the 45th day the fibrous capsule of kidney is reduced, the cortex becomes thinner, nephrons and renal pyramid are deformed. The structure of cardiomyocytes is damaged. It shows areas of cytolysis and fragmentation of muscle fibers. In the stroma of the myocardium is proliferation of connective tissue. Conclusions. 1. Method of administration and dose of alloxan are adequate to induce hyperglycemia and survival of animals. 2. At early stages of the experiment, hemocirculation disorders predominate in the organs under study. 3. In the later stages of the experiment, we found the deformation of nephrons and pyramids, damage of cardiomyocytes and local stromal fibrosis of the myocardium. 4. Under conditions of alloxan hyperglycaemia, the morphological transformations of the kidney are more pronounced in comparison with myocardial changes.