Abstract
Objective: proteomic analysis of blood serum of children with bronchial asthma with a history of indication of the pathology of the perinatal period.Materials and methods: a comprehensive examination of children suffering from asthma and patients of the control group using proteomic analysis of blood serum was carried out. Proteomic analysis of blood serum was performed using two-dimensional high-resolution gel electrophoresis followed by staining of gels with fl uorescent dye and time-of-fl ight MALDI mass spectrometry. The proteins were identifi ed using Mascot MS peptide fi ngerprint analysis algorithm and Swiss-Prot protein database.Results: comparative analysis of the proteomic profi le of blood serum of children with pathology of the central nervous system in the perinatal period and developed in the subsequent bronchial asthma and control group patients allowed to establish that number proteins products were reduced in this disease. The proteins in the molecular mass range 16-33 kDa (p<0.05) were identifi ed: glutathione peroxidase 3, transthyretin and system components compliment С4В and C3.Conclusion: modifi cation of the production of these proteins indicates the presence of damage at the molecular level, leading to metabolic and functional disorders in the body of a patient with asthma having a history of indicating the pathology of the perinatal period.
Highlights
Цель: протеомный анализ сыворотки крови детей с бронхиальной астмой (БА), имеющих в анамнезе указание на патологию перинатального периода
Materials and methods: a comprehensive examination of children suffering from asthma and patients of the control group using proteomic analysis of blood serum was carried out
Proteomic analysis of blood serum was performed using two-dimensional high-resolution gel electrophoresis followed by staining of gels with fluorescent dye and time-of-flight MALDI mass spectrometry
Summary
Особенности протеомного профиля сыворотки крови детей с бронхиальной астмой, перенесших перинатальное поражение центральной нервной системы. Цель: протеомный анализ сыворотки крови детей с бронхиальной астмой (БА), имеющих в анамнезе указание на патологию перинатального периода. Результаты: сравнительный анализ протеомного профиля сыворотки крови детей, перенесших патологию ЦНС в перинатальным периоде и с развившейся в последующем БА, и пациентов контрольной группы позволил установить, что продукция ряда белков снижена при указанной патологии. Objective: proteomic analysis of blood serum of children with bronchial asthma with a history of indication of the pathology of the perinatal period. Results: comparative analysis of the proteomic profile of blood serum of children with pathology of the central nervous system in the perinatal period and developed in the subsequent bronchial asthma and control group patients allowed to establish that number proteins products were reduced in this disease. Conclusion: modification of the production of these proteins indicates the presence of damage at the molecular level, leading to metabolic and functional disorders in the body of a patient with asthma having a history of indicating the pathology of the perinatal period
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